ABSTRACT
El consumo de hortalizas crudas representa un vehículo importante en la transmisión delas parasitosis intestinales. Entre enero y abril de 2006, se evaluaron parasitológicamente mediante lavado con agua destilada estéril, sedimentación espontánea por 24 horas, centrifugación [331de fuerza centrífuga relativa (FCR) x g, por 3-5 minutos] y la observación microscópica de tinciones de Lugol y Kinyoun, 127 muestras de vegetales correspondientes a 10 especies de hortalizas, las cuales se obtuvieron al azar en mercados y supermercados de la ciudad de Coro, estado Falcón, Venezuela. Se detectó una prevalencia global del 32,28% (41/127), siendo el apio españa (100%), el repollo (64,29%) y la lechuga (44,44%) las hortalizas que presentaron mayores porcentajes de contaminación parasitaria. Los parásitos intestinales más frecuentemente observados fueron: Ascaris sp. (11,81%) y los coccideos intestinales Cyclospora sp. (8,66%) y Cryptosporidium sp.(5,51%). No se encontró una relación estadísticamente significativa entre tipo de establecimiento(público o privado) y los porcentajes de contaminación (P > 0,05). En relación con el sitio de origen del cultivo, sólo se hallaron diferencias estadísticamente significativas entre los porcentajes globales de hortalizas contaminadas provenientes de la región andina y la falconiana (c2=7,61; P= 0,01). Se sugiere que las hortalizas y vegetales expendidos en los mercados y supermercados de la ciudad de Coro pueden potencialmente jugar un papel significativo en la diseminación de enfermedades parasitarias vehiculizadas por alimentos. Se recomienda la fiscalización sanitaria permanente de los procesos pre y post cosecha, y la implementación obligatoria de los exámenes coproscópicos a los manipuladores de hortalizas
The consumption of raw vegetables is amajor way in the transmission of intestinal parasiticinfections. Between January and April 2006, a total of127 samples from 10 different vegetable species wererandomly selected from markets and supermarketslocated in Coro, Falcon state, Venezuela. Botanicalsamples were washed with sterile distillated waterallowing sedimentation at room temperature for 24h.Five mL of sediment were centrifuged at 331 relativecentrifuge force (RCF) x g for 3-5min. Sediment wasexamined in lugol and Kinyoun stained slides throughlight microscopy. The global prevalence of the parasiteswas 32.28% (41/127), with higher contaminationpercentages for celery (100%), cabbage (64.29%)and lettucee (44.44%). Ascaris sp. (11.81%) and theintestinal coccidians Cyclospora sp. (8.66%) andCryptosporidium sp. (5.1%) were the most commonisolated parasites. There was not found a statisticallysignificant association between type of trading local(private or popular) and contamination frequencies(P > 0.05). In relation to crop origin, a statisticallysignificant difference was only detected between globalpercentages of contaminated vegetables coming fromAndean and Falcon state regions (c2=7.61; P= 0.01).The potential role of vegetables in the transmissionof foodborne parasitic infections in Coro city, Falconstate, Venezuela, is suggested. A continuous sanitaryprocedure during the pre-and post-harvest crop processand an obligatory implementation of coproscopictesting for vegetable handlers, is recommended
Subject(s)
Humans , Male , Female , Food Parasitology , Intestinal Diseases, Parasitic/microbiology , Sanitary Profiles , Vegetables , Health SurveillanceABSTRACT
Com a finalidade de verificar a frequência das parasitoses intestinais que mais incidem na cidade de Florianópolis, foram analisadas, de fevereiro a junho de 1996, 1289 amostras fecais de pacientes atentidos no Hospital Universitário da UFSC. Para o diagnóstico das enteroparasitoses foram empregados os métodos de Ritchie, de Hoffman e cols e de Baermann-Moraes...
Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Adult , Intestinal Diseases, Parasitic/microbiology , Outpatients/psychology , PrevalenceABSTRACT
Intestinal amebiasis is still an important health problem in developing regions of the world. In order to advance our knowledge on the pathogenesis and to test the relevance of recently obtained in vitro observations, suitable in vivo experimental models of intestinal amebiasis are needed. In the past a variety of laboratory animals have been used, but the mouse, whose genetic and immunology is well known, has been seldomly used. Therefore, Entamoeba histolytica strain HM1:IMSS was directly inoculated into the cecum of C3H/HeJH mice, which were sacrified at 5, 10, 15, 20, 25 and 30 days for histopathologic analysis. An ulcerative inflammatory disease highly reminiscent of human amebiasis was observed. Early 5 day lesions consited of tiny erosions of the surface epithelium which evolved to deeper and more extensive destructive lesions of the cecal wall. Indeed, flask-shaped ulcers, intestinal perforations and intramural abscess formation were observed at later time. It was noticeable that, depite the lack of obvious significant tissue invasion by amebae, ulcerative disease was extensive and found virtually in all mice. These observations support the view that tissue invasion by trophozoites is not necessarily required for ulcerative disease to occur, suggesting a role for toxic factors released by amebae
Subject(s)
Mice , Animals , Amebiasis/parasitology , Amoeba/pathogenicity , Entamoeba histolytica/pathogenicity , Intestinal Diseases, Parasitic/microbiology , Intestinal Diseases/physiopathologyABSTRACT
De 13.196 coproculturas realizadas durante o triênio 1978-1980 em Recife, Pernambuco, foram isoladas 1.720 salmonelas, das quais 1.387 foram caracterizadas sorologicamente. O estudo global possibilitou o reconhecimento de 63 sorotipos concentrados em primeiro plano no grupo sorológico B (73,18%) e identificando-se como tipos mais incidentes: S. typhimurium, S. saint-paul, S. poona, S. derby, S. agona, S. newport, S. oranienburg, S. infantis, S. tshiongwe e S. ndolo, que representaram 1.231 amostras ou 88,75% do total de isolamentos. Algumas consideraçöes de ordem epidemiológica e bacteriológica säo discutidas em relaçäo aos quatros sorotipos mais freqüentes